STUDYING HOW THE GENOME
REGULATES COMPLEX SYSTEMS,
ONE CELL AT A TIME.




 

Research

Our lab is interested in understanding how the human genome regulates itself to bring about all of the cellular diversity present in our bodies. In addition, we are interested in how genetic variation and environmental exposures in human populations impact that regulation and sometimes lead to complex disease. The particular disease model that we focus on is asthma, a complex disease affecting ~10-20% of the population. The reasons we think that single-cell approaches would be particularly applicable to asthma are: (1) the disease involves complex interactions between many cell types of the lung and immune system that are difficult to model; And (2) there are several exquisitely detailed examples of gene-environment interactions that influence asthma outcomes many years later, but we still know relatively little about the molecular mechanisms that lead from exposure to disease. To study these phenomena, we use single-cell genomics technologies so that we can evaluate the impact of genetic and environmental variability from the perspective of whole tissues rather than having to isolate individual cell types or use simplistic cellular models. Working at the nexus of functional genomics, computational biology, and cellular biology, our group is both experimental and computational and often has to develop novel technologies or methods to address our research questions.

News

August 26, 2019: Noura AlDarwish and David Jones start rotations in the lab. Welcome Noura and David!

June 10, 2019: Holly Welfley starts a rotation in the lab. Welcome Holly!

January 10, 2019: Selina Kindelay starts a rotation in the lab. Welcome Selina!

November 5, 2018: Michael Rice starts in the lab. Welcome Mike!

October 22, 2018: Hao Zhang starts a rotation in the lab. Welcome Hao!

August 2, 2018: Darren is first author on a paper published in Cell. See coverage here and here. You can access the paper here.

June 18, 2018: We opened our doors in the Biosciences Research Labs at UofA!

People

Dr. C

Darren Cusanovich

Research Assistant Professor, Cellular and Molecular Medicine (CMM)
Assistant Research Scientist, Asthma & Airway Disease Research Center (A2DRC)

Darren received his B.S. in Music Busines (yes, Music Business!!) from Loyola University New Orleans in 2002. After a brief stint in the music industry in Los Angeles, he realized that his true passion was genomics (it's a long story). So, Darren spent a few years in Donata Vercelli's Lab here at the University of Arizona - his first exposure to asthma genomics research. Darren then got his Ph.D. in Human Genetics from The University of Chicago (in Yoav Gilad's lab). While there, Darren studied how genetic variation in human populations perturbs gene expression and can lead to complex disease susceptibilities. Darren then did his postdoctoral research in Jay Shendure's lab at the University of Washington. While in Seattle, he developed a novel single-cell chromatin accessibility assay and applied it to several model systems to study the variation in gene regulatory landscapes present in complex tissues.

In 2018, Darren accepted a position here at the UofA in CMM and the A2DRC.

darrenc@email.arizona.edu
CV
Pubmed



Michael Rice

Michael Rice

Research Scientist, Senior
mrice@email.arizona.edu



Holly Welfley

Holly Welfley

Graduate Student (CBIO)
hwelfley@email.arizona.edu



Hao Zhang

Hao Zhang

Graduate Student (GPMM)
haozhang1@email.arizona.edu



You

You?

Looking for an exciting opportunity? Do you like the idea of pursuing cutting-edge genomics research? Do you like the idea of living in a place with lots of sunshine, lots of opportunity for outdoor activities, vibrant southwestern culture, easy commutes, and an affordable cost living? E-mail us! We are always looking for talented, enthusiastic people to work with.



**We are always open to having talented people join the team and help drive our research program. If you're interested in being a part of our group (at any level) feel free to drop us a line.**

Publications

"*" denotes equal contribution.



2018

Allan CM, Heizer PJ, Tu Y, Sandoval NP, Jung RS, Morales JE, Sajti E, Troutman TD, Saunders TL, Cusanovich DA, Beigneux AP, Romanoski CE, Fong LG, Young SG. An upstream enhancer regulates Gpihbp1 expression in a tissue-specific manner. Journal of Lipid Research. 2018 Dec 31; pii: jlr.M091322. [journal]


Cao J, Cusanovich DA, Ramani V, Aghamirzaie D, Pliner HA, Hill AJ, Daza RM, McFaline-Figueroa JL, Packer JS, Christiansen L, Steemers FJ, Adey AC, Trapnell C, Shendure J. Joint profiling of chromatin accessibility and gene expression in thousands of single cells. Science. 2018 Sep 28; 361(6409):1380-1385. [journal]


Pliner HA, Packer JS, McFaline-Figueroa JL, Cusanovich DA, Daza RM, Aghamirzaie D, Srivatsan S, Qiu X, Jackson D, Minkina A, Adey AC, Steemers FJ, Shendure J, Trapnell C. Cicero Predicts cis-Regulatory DNA Interactions from Single-Cell Chromatin Accessibility Data. Molecular Cell. 2018 Sep 6;71(5):858-871.e8. [journal]


Cusanovich DA*, Hill AJ*, Aghamirzaie D, Daza RM, Pliner HA, Berletch JB, Filippova GN, Huang X, Christiansen L, DeWitt WS, Lee C, Regalado SG, Read DF, Steemers FJ, Disteche CM, Trapnell C, Shendure J. A Single-Cell Atlas of In Vivo Mammalian Chromatin Accessibility. Cell. 2018 Aug 23; 174(5):1309-1324.e18. [journal]


Cusanovich DA*, Reddington JP*, Garfield DA*, Daza R, Aghamirzaie D, Marco-Ferreres R, Pliner HA, Christiansen L, Qiu X, Steemers FJ, Trapnell C, Shendure J, Furlong EEM. The cis-regulatory dynamics of embryonic development at single-cell resolution. Nature. 2018 Mar 22; 555(7697):538-542. [journal]




2017

Cao J, Packer JS, Ramani V, Cusanovich DA, Huynh C, Daza R, Qiu X, Lee C, Furlan SN, Steemers FJ, Adey A, Waterston RH, Trapnell C, Shendure J. Comprehensive single-cell transcriptional profiling of a multicellular organism. Science. 2017 Aug 18; 357(6352):661-667. [journal]


Gasperini M, Findlay GM, McKenna A, Milbank JH, Lee C, Zhang MD, Cusanovich DA, Shendure J. CRISPR/Cas9-Mediated Scanning for Regulatory Elements Required for HPRT1 Expression via Thousands of Large, Programmed Genomic Deletions. American Journal of Human Genetics. 2017 Aug 3; 101(2):192-205. [journal]




2016

Ramani V, Cusanovich DA, Hause RJ, Ma W, Deng X, Blau CA, Disteche CM, Noble WS, Shendure J, Duan Z. Mapping 3D genome architecture through in situ DNase Hi-C. Nature Protocols. 2016 Nov; 11(11):2104-2121. [journal]


Cusanovich DA, Caliskan M, Billstrand C, Michelini KJ, Chavarria C, De Leon S, Mitrano A, Lewellyn N, Elias JA, Chupp GL, Lang RM, Shah SJ, DeCara JM, Gilad Y, Ober C. Integrated Analyses of Gene Expression and Genetic Association Studies in a Founder Population. Human Molecular Genetics. 2016 Feb 29; 25(10):2104-2112. [journal]




2015

Davenport ER, Cusanovich DA, Michelini K, Barreiro LB, Ober C, Gilad Y. Genome-Wide Association Studies of the Human Gut Microbiota. PLoS One. 2015 Nov 3; 10(11):e0140301. [journal]


Cusanovich DA, Daza R, Adey A, Pliner H, Christiansen L, Gunderson KL, Steemers FJ, Trapnell C, Shendure J. Multiplex single-cell profiling of chromatin accessibility by combinatorial cellular indexing. Science. 2015 May 22; 348(6237):910-914. [journal]




2014

Cusanovich DA, Pavlovic B, Pritchard JK, Gilad, Y. The functional consequences of variation in transcription factor binding. PLoS Genetics. 2014 Mar 6; 10(3):e1004226. [journal]




2013

Khan Z, Ford MJ, Cusanovich DA, Mitrano A, Pritchard JK, Gilad Y. Primate transcript and protein expression levels evolve under compensatory selection pressures. Science. 2013 Nov 29; 342(6162):1100-1104. [journal]




2012

Iskow RC, Gokcumen O, Abyzov A, Malukiewicz J, Zhu Q, Sukumar AT, Pai AA, Mills RE, Habegger L, Cusanovich DA, Rubel MA, Perry GH, Gerstein M, Stone AC, Gilad Y, Lee C. Regulatory element copy number differences shape primate expression profiles. Proceedings of the National Academy of Sciences. 2012 Jul 31; 109(31):12656-61. [journal]


Cusanovich DA, Billstrand C, Zhou X, Chavarria C, De Leon S, Michelini K, Pai AA, Ober C, Gilad Y. The combination of a genome-wide association study of lymphocyte count and analysis of gene expression data reveals novel asthma candidate genes. Human Molecular Genetics. 2012 May 1; 21(9):2111-23. [journal]




2011

Caliskan M, Cusanovich DA, Ober C, Gilad Y. The effects of EBV transformation on gene expression levels and methylation profiles. Human Molecular Genetics. 2011 Apr 15; 20(8):1643-52. [journal]




2009

Ober C, Nord AS, Thompson EE, Pan L, Tan Z, Cusanovich D, Sun Y, Nicolae R, Edelstein C, Schneider DH, Billstrand C, Pfaffinger D, Phillips N, Anderson RL, Philips B, Rajagopalan R, Hatsukami TS, Rieder MJ, Heagerty PJ, Nickerson DA, Abney M, Marcovina S, Jarvik GP, Scanu AM, Nicolae DL. Genome-wide association study of plasma lipoprotein(a) levels identifies multiple genes on chromosome 6q. Journal of Lipid Research. 2009 May; 50(5):798-806. [journal]

Contact

Asthma & Airway Disease Research Center
University of Arizona Biosciences Research Labs, Room 421
1230 N Cherry Avenue
Tucson, AZ 85721
Office: 520-626-8639
Lab: 520-626-6862
darrenc@email.arizona.edu

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